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| Cavalcanti,Lilian Cristina Gomes; Moraes,José Carlos Ferrugem; Faria,Danielle Assis de; McManus,Concepta Margaret; Nepomuceno,Alcebiades Renato; Souza,Carlos José Hoff de; Caetano,Alexandre Rodrigues; Paiva,Samuel Rezende. |
| Abstract: The objective of this work was to identify single nucleotide polymorphisms (SNPs) in resequencing data from MC1R, ASIP, and TYRP1 genes derived from Crioula sheep (Ovis aris) with different coat colors. Polymorphisms in the ASIP (agouti-signaling protein), MC1R (melanocortin 1 receptor), and TRYP1 (tyrosinase-related protein 1) genes were analyzed in 115 sheep from Embrapa’s conservation nucleus of crioula sheep, in Brazil. A total of 7,914 bp were sequenced per animal, and 14 SNPs were identified. Two additional assays were performed to detect duplications and deletions in the ASIP gene. Ninety-five percent of the coat color variation was explained by epistatic interactions observed between specific alleles in the MC1R and ASIP genes. Evidence... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ovis aris; ASIP; Genetic resources conservation; Marker-assisted selection; MC1R; TYRP1. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2017000800615 |
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| Silva,Elizabete Cristina da; Dutra Junior,Wilson Moreira; Ianella,Patrícia; Gomes Filho,Manoel Adrião; Oliveira,Cláudio José Parro de; Ferreira,Débora Nathália de Moura; Caetano,Alexandre Rodrigues; Paiva,Samuel Rezende. |
| This study estimated the genetic diversity and structure of 12 genetic groups (GG) of locally adapted and specialized pigs in the state of Pernambuco using 22 microsatellite markers. Nine locally adapted breeds (Baé, Caruncho, Canastra, Canastrão, Mamelado, Moura, Nilo, Piau and UDB (Undefined Breed)) and 3 specialized breeds (Duroc, Landrace and Large White), totaling 190 animals, were analyzed. The Analysis of Molecular Variance (AMOVA) showed that 3.2% of the total variation was due to differences between genetic groups, and 3.6% to differences between local and commercial pigs. One hundred and ninety eight alleles were identified and apart from the Large White breed, all GG presented Hardy-Weinberg Equilibrium deviations for some loci. The total and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Animal genetics resources; Genetic variability; Microsatellites loci; Sus Scrofa. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982011000800010 |
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| Rebelato,Arnaldo Basso; Caetano,Alexandre Rodrigues. |
| Abstract: Runs of homozygosity (ROHs) are long stretches of homozygous genomic segments, identifiable by molecular markers, which can provide genomic information for accurate estimates to characterize populations, determine evolutionary history and demographic information, estimate levels of consanguinity, and identify selection signatures in production animals. This review paper aims to perform a survey of the works on the efficiency of ROHs for these purposes. Factors such as genetic drift, natural or artificial selection, founder effect, and effective population size directly influence the size and distribution of ROHs along the genome. Individually, genome estimates of consanguinity based on ROHs can be obtained using the FROH index, which is generally... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Autozygosity; FROH; Genotyping; Selection signatures; Single nucleotide polymorphism; SNPs. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000900975 |
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| Capdeville,Guy de; Souza Júnior,Manoel Teixeira; Santos,Jansen Rodrigo Pereira; Miranda,Simoni Paula; Caetano,Alexandre Rodrigues; Falcão,Rosana; Gomes,Ana Cristina Menezes Mendes. |
| The objective of this work was to investigate possible modes of action of the yeast Cryptococcus magnus in controlling anthracnose (Colletotrichum gloeosporioides) on post harvested papaya fruits. Scanning electron microscopy was used to analyze the effect of the yeast on inoculations done after harvest. Results showed that C. magnus is able to colonize wound surfaces much faster than the pathogen, outcompeting the later for space and probably for nutrients. In addition, C. magnus produces a flocculent matrix, which affects hyphae integrity. The competition for space and the production of substances that affect hyphae integrity are among the most important modes of action of this yeast. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Carica papaya; Anthracnose; Antagonist yeast; Biological control; Competition. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2007001100004 |
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| Paim,Tiago do Prado; McManus,Concepta; Vieira,Fábio Danilo; Oliveira,Stanley Robson de Medeiros; Facó,Olivardo; Azevedo,Hymerson Costa; Araújo,Adriana Mello de; Moraes,José Carlos Ferrugem; Yamagishi,Michel Eduardo Beleza; Carneiro,Paulo Luiz Souza; Caetano,Alexandre Rodrigues; Paiva,Samuel Rezende. |
| Abstract: The objective of this work was to evaluate the usefulness of a subset of 18 single nucleotide polymorphisms (SNPs) for breed identification of Brazilian Crioula, Morada Nova (MN), and Santa Inês (SI) sheep. Data of 588 animals were analyzed with the Structure software. Assignments higher than 90% confidence were observed in 82% of the studied samples. Most of the low-value assignments were observed in MN and SI breeds. Therefore, although there is a high reliability in this subset of 18 SNPs, it is not enough for an unequivocal assignment of the studied breeds, mainly of hair breeds. A more precise panel still needs to be developed for the widespread use in breed assignment. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ovis aries; Animal genetic resources; Certification of origin; Genomics; Traceability. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2019000104302 |
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| Silva,Elizabete Cristina da; McManus,Concepta Margaret; Guimarães,Maria Pia Souza Lima Mattos de Paiva; Gouveia,Aurora M.G.; Facó,Olivardo; Pimentel,Daniel M.; Caetano,Alexandre Rodrigues; Paiva,Samuel Rezende. |
| Brazilian goats are generally kept in small herds and extensive rearing systems, mainly in the northeastern region of the country. Despite production improvement in recent years, the lack of pedigree control has affected genetic progress. This study aimed to validate a panel of 16 microsatellites for parentage testing in locally adapted and commercial goats breeds raised in Brazil, as well as to compare its efficiency with the panel recommended by the Brazilian Ministry of Agriculture, Livestock and Supplies (MAPA) in 2004. The number of alleles and expected heterozygosity (He) per marker ranged from four to 18, and from 0.051 to 0.831, respectively. Using all markers, 100% of parentage cases of the validation dataset were resolved with a strict confidence... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Parentage errors; Animal breeding; Animal genetic resources; Capra hircus. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100010 |
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